Begoña Roibás da Torre
Experimental skill summary:
o Bioinformatics Tools: Primer design, sequence analysis, blast searches (Gene Fisher - Interactive PCR Primer Design, Primer-BLAST, Entrez Gene, VecScreen , Entrez Genomes , COGs - Clusters of Orthologous Groups , Entrez Genomes),ApE plasmid editor.
o Maintenance of laboratory records through Excel databasing and Laboratory Integrated Management Systems (LIMS).
o Genomic, Plasmid and Bacterial DNA extraction.
o Isolate DNA from tissue, cells and blood, prepare samples for PCR and sequencing, and sequence editing.
o RNA extraction.
o PCR, RT-PCR, Q-PCR, TouchDown PCR.
o Gel electrophoresis and restriction analysis.
o Chromatography: TLC, HPLC, Columnm Chromatography.
o Dot-blot hybridization.
o Genotyping: transgenic mouse lines, human patients, MHC genotyping for human transplants.
o Microsatellite analysis.
o Gene cloning: TOPO cloning, gateway cloning .High experience in the use of restriction enzymes..
o Bacterial culture .
o Bacterial cloning; transformation, electroporation and transfection.
o Established a cell culture: Tissue culture of mouse ES and iPS cells; for screening of the presence of the transgene, maintenance passaging, thawing, long term cryostorage and LacZ staining. Cell counter to determine to determine cells concentration.
o Established of a primary culture, manteinance passaging, thawing, long term cryostorage.
o Transfection of cell lines.
o Established human feeder cell lines and maintained by microscope observation.
o Human Blood analysis .
Professional Experience:
Ø Currently work: Researcher Associate:
o The laboratory of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University (February 2010 to present)
-Gene cloning in different experssion vectors.
-T cloning and Gateway cloning.
-Generation of 2A peptide-linked multicistronic vectors.
-Culture of mouse cells (CF-1, STO) and feeder cells preparation.
-Primary culture and transfection.
-Basic molecular work (DNA and RNA extraction from blood,tissue and cells)
Ø Volunteer Researcher:
o The laboratory of Dr. Andras Nagy, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto ON (March 2009 to September 2009)
-Genotype mouse samples (DNA extraction, PCR, gel electrophoresis), working side by side with many graduate students and post-doctoral researchers.
-Culture of mouse iPS cells for screening of the presence of the transgene, maintenance, passaging, long term cryostorage, LacZ staining.
-Genotyping mouse iPS cells (transposon screening).
-Concurrent with intensive English as a Second language classes.
Ø Paid internship:
o The Estacion Experimental Del Zaidin (Spanish National Research Council- CSIC )Granada ,Spain (July to December 2007)
-Study of Circadian Rhythms in Pseudomonas Putida.
- Work with bioinformatics tools, and different techniques in Molecular Biology and Microbiology, such as Bacteria isolation from different surfaces (ground, plants) bacterial culture, bacterial cloning, bacterial transfection, and chromosomic DNA and plasmid purification, restriction analysis.
Ø Internships:
o Institute of Parasitological and Biomedicine Lopez-Neira (IPBLN). Consejo Superior de Investigaciones Cientificas (Spanish National Research Council-CSIC), Granada, Spain. July to August, 2006.
-Genotyping and analysis by Q-PCR of patient samples of GCA (Giant Cells Arteritis) as well as of Chron’s patients
-Designing of primers for genotyping using bioinformatics tools (GeneFisher - Interactive PCR Primer Design, Primer-BLAST) and analysis of sequences.
-Genotyping and analysis by Q-PCR of patient samples of GCA (Giant Cells Arteritis) as well as of Chron’s patients
-Designing of primers for genotyping using bioinformatics tools (GeneFisher - Interactive PCR Primer Design, Primer-BLAST) and analysis of sequences.
o Governmental project, Virgen Del Rocio Hospital (Immunology Service) Seville , Spain
- Genetic analysis of patients’ samples (Rheumatoid Arthritis, Lupus, Chron, Ulcerous Colitis and Indeterminate Colitis) by DNA extraction and analysis (PCR-ARMS and restriction digestion) and gene expression analysis by RNA extraction and quantitative RT-PCR (Taqman).
-Routine work in the laboratory (Control of entry and storage of samples, extractions, PCR, analysis, maintenance of laboratory databasing).
o Company BIOMIG S.A, Hospital POLUSA. Lugo , Spain
-Routine haematological analysis of human blood samples to determinate for example glucose, protein and hormone levels in a laboratory of clinical analysis and special biochemistry.
- Additional duties included quality controls and bacteriological analysis.
- Additional duties included quality controls and bacteriological analysis.
o Laboratory of Enteric Virus of the Department of Biology. University of Santiago de Compstela , Spain
-Samples processing, DNA isolation, PCR.
-Cellular culture and analysis and detections of enterovirus on molluscs (dot-blot hybridization, bacteriophage detection and RT-PCR techniques).
o Unit of Molecular Medicine of the Galician Foundation of Genomic Medicine (Clinical University Hospital ) Santiago de Compostela , Spain
-Collection of samples, quantification and storage of DNA, analysis of fragments and microsatellites for electrophoresis, DNA sequence and primer design.
-Routine activities of maintenance of the hospital unit: Preparation of buffer solutions, reactive aliquotation, pour gels, autoclaving solutions.
Ø Publications in international magazines:
o Rueda B, Roibas B, Martin J, Gonzalez-Gay MA.
Influence of IL-10 promoter polymorphism in the susceptibility to giant cell arteritis in North-western Spain.
J Rheumatol. 2007 Jul; 34(7):1535-9. Epub 2007 Jun. 1.
o Soriano MI, Roibás B, García AB, Espinosa-Urgel M.
Evidence of circadian rhythms in non-photosynthetic bacteria?
J Circadian Rhythms. 2010; 8:8. Epub 2010 Sept 16.
o M. Saadeldin ,B.H. Kim, B. Roibas B,O.J. Koo ,G. Jang ,B.C. Lee.
Isolation of bovine trophoblast and its reprogramming by nuclear transfer.
Molecular Reproduction and Development (MRD). Reprod Fertil Dev. 2011 Jan;23(1):134-135.
Ø Congress and International Meetings:
o Education, Research and Development Day (ERD-Day). Seoul National University (SNU) 2010.
Reprogramming of bovine trophectoderm karyoplast by nuclear transfer
Islam Mohamed Saadeldin*, BongHan Kim, Roibas B, OKJae Koo, Goo Jang,ByeongChun Lee.
o 37th IETS Internacional Meeting ,Florida,USA.
Isolation of bovine trophoblast and its reprogramming by somatic cell nuclear transfer.
o 2nd Joint Workshop between College of Veterinary Medicine, Seoul National University and United Graduate School of Veterinary Sciences, Gifu UniversityFebruary 20, 2011.
Two abstracts:
1)Production of porcine cloned embryos derived from conditionally exogenous gene expressing cells using Cre-LoxP.
JoonHo Moon, SuJin Kim, HeeJung Park, JungTaek Kang, SolJi Park, OkJae Koo, Roibas B, ByeongChun Lee, Goo Jang.
2)IVF- or NT-derived trophoblast culture: An easy model for revealing interferon tau epigenetics and transgenesis.
Islam Mohamed Saadeldin, BongHan Kim, Roibas B, OKJae Koo,Goo Jang, ByeongChun Lee.
Ø Education:
B.Sc in Biochemistry. The University of Granada.2006-2008
-Courses: Cell biology, Functional Genomics, Biochemistry, Developmental Biology,Industrial Microbiology,Bioinformatic.
B.Sc in Biology speciality in Molecular Biology and Biotechnology. The University of Santiago de Compostela , Spain
-Courses: Genetic engineering and Biotechnology, Molecular Genetics, Recombinant DNA technology, Animal and Plant Physiology, Metabolic Regulation, Clinical Biochemistry and Molecular Pathology, Animal Microscopic Organography.
Ø Language courses:
Course of Intensive English with 20 lessons per week from July 14 to September 5, 2003 in ST Gilles College, Eastbourne (United Kingdom
The course included Speaking, Listening, Reading
Course of intensive English with 30 lessons per week from January 26 to July 6, 2009 in ILAC School , Toronto (Canada
The course included Speaking, Listening, Reading
Korean evening course at the Language Education Institute, Seoul National University. Level 1 and 2 completed. Currently level 3.
